Advantages and disadvantages of thin and thick blood smear

Why thick blood protects from a heart attack sciencedaily. Red blood cells have the greatest influence on the blood s viscosity, since they account for up to half its volume. Any laboratory that can perform routine hematology tests is equipped to perform a thin and thick malaria smear. Thin blood smears assists specialists with discovering what types of malaria is creating the disease. Submit all blood smears, perfect or otherwise, as areas of the film may be suitable for examination. During a blood smear, laboratory technicians will examine three main components of blood. The direct microscopic visualization of the malarial parasite on the thick andor thin blood smears has been the gold standard for malaria diagnosis.

A comparative study of thick and thin blood films in the. What is the disadvantage of having a really thick smear. Thin blood smear for demonstration and differentiation of leukocytes. This video shows the procedure for making thick and thin blood smear. Difference between thick blood smears and thin blood smears. However, if blood is too thin due to overuse of anticoagulatory medications, those might be at risk for another side. Procedure for thick blood film staining technique a thick film was made by placing a large drop of blood about 15mm in size on the centre of greasefree microscopic slide. The blood clot usually occurs in a persons vein, which can cause pain and affect circulation in and around. Blood smear technique for veterinarians agriculture and food. Although rapid diagnostic and molecular tests for malaria are increasing in prevalence and importance, the standard method for malaria diagnosis in much of the world remains the examination of thick and thin blood films.

Seed haematology the role of the peripheral blood smear in the modern haematology laboratory 2 sysmex educational enhancement and development february 20 the viscosity haematocrit of the blood, which can be highly variable from patient to patient, will also affect the smear. Thin blood smears have the advantages to achieve a better optical differention in the morphology of intracellular parasitic formsstages as haemogragarines in erythrozytes. Thus, thick smears allow a more efficient detection of parasites increased sensitivity. Jul 20, 2016 video tutorial on how to perform a proper thick and thin smear for malaria diagnosis by stephane proux, malaria diagnosis lab supervisor at shoklo malaria research unit smru. After thin and thick blood smears were prepared, a few drops. A potential complication of thick film preparations is sloughing of the blood droplet from the slide during staining or rinsing, resulting in the loss of sample. In case of a blood check up, i would make a thick smear first to assess the general constitution and the potential. It is generally used to differentiate between and identify white blood cells, malaria parasites, and. Thick and thin blood smears will let doctors know the percentage of red blood cells that are infected.

Im trying to think of a moviemovie scene and all i can remember is a kid that is saying our lord in heaven, hallowed be thy name. If one test is negative and no parasites are found, you will have repeated blood smea. Advantages and disadvantages of blood film thick film. Thick blood smears are most useful for detecting the presence of parasites, because they examine a larger sample of blood. A blood filmor peripheral blood smearis a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various. Objectives the purpose of thick and thin blood film procedures of thick and thin blood film stains used for thick and thin blood film microscopic features of thick and thin blood film.

Dec 30, 2019 i think and im sure, for practicing it that doing an ihc on a blood smear is not a good idea. The extra time and care taken during the field season will be rewarded later when the smears. Leishman stain, also known as leishmans stain, is used in microscopy for staining blood smears. The basophilia is not reliably detected by an automated counter and therefore requires clarification by examination of a blood smear. Direct smear microscopy is relatively insensitive as at least 5,000 bacilli per millilitre of sputum are required for direct microscopy to be positive. Thin blood vs thick blood, is one better than the other. A blood smear is a blood test used to look for abnormalities in blood cells. Learn about the different types of tests doctors use to test for malaria in your body and how the exam is performed. Thin blood smears helps to discover which species of parasite is causing the infection.

The extra time and care taken during the field season will be rewarded later when the smears must be scanned, and parasites identified and counted. Thick blood smear for diagnosis of blood protozoan parasites and blood abnormalities eg anemiae. Oct 28, 2017 thick blood or hypercoagulability is a condition where the blood is thicker and stickier than usual. Hence, false negativity is frequent and requires two or more repetitions that may not be well appreciated by patients. The sensitivity is extremely low, and the main use of thin smears is in fact the specific identification of trypanosomes found in wet or thick smears.

Cdc malaria malaria worldwide how can malaria cases and. The thin peripheral blood smear is made by placing a wellmixed drop of blood 1 to. A method for reducing the sloughing of thick blood films. Thick smears were interpreted as negative only after the entire smear was examined maximum, 30 min. Comparison of blood smear, antigen detection, and nested. Seed haematology the role of the peripheral blood smear. The thick blood smear has a better rate of parasite detection. The thin smear technique has no false positivity but it is not very sensitive. Introduction, objectives, thick smear procedure, thin smear procedure, application of smears, advantages and limitations of blood smears. A thin blood smear is a drop of blood that is spread across a large area of the. Thick blood smear for diagnosis of blood protozoan parasites and blood. In 1954, kato and miura were the first tointroduce a new method, the cellophanethick smear technique which involved a principle of direct fecal sampling kato and miura,1954. Comparative study of peripheral blood smear, quantitative buffy coat. A method for reducing the sloughing of thick blood films for.

Doctors use thick and thin blood smears to determine whether you have malaria. The gold standard for malaria diagnosis is the examination of thick and thin blood films. See dpdx specimen preparation a blood specimen collected from the patient is spread as a thick or thin blood smear, stained with a romanovsky stain most often giemsa, and examined with a 100x oil immersion objective. Volunteers were treated when a single parasite was seen by blood film, after the appearance of the parasite was confirmed by a second microscopist. At least 100 high powered fields of a thick blood film were viewed and quantitative pcr performed on each sample. These techniques have their own advantages and disadvantages with. Thick blood smears are most useful for detecting the presence of parasites because they examine a larger sample of blood.

Sep 17, 2015 a thin blood smear is usually a drop of blood that is widening over an extensive range of the slide. Microscopy, while laborious and technically challenging, persists through its combined advantages. When a person has hypercoagulability, they are prone to excess blood clots. While rare, one of the more common reasons for thick blood is called polycythemia vera pv, in which the body produces excessive blood cells mostly too many red blood cells. Microscopy of thick blood films is the usual diagnostic test for plasmodium falciparum malaria.

Thick smears consist of a thick layer of dehemoglobinized lysed red. Thick blood film examination for plasmodium falciparum. A lab technician, doctor, or nurse will take some of your blood and send it to a lab to be stained to make any. For pbs, two observers examined the smear under 100x oil immersion magnification for a maximum period of 10 min each, before calling the smear. Aug 25, 2016 this video shows the procedure for making thick and thin blood smear. The blood elements including parasites, if any are more concentrated app. Thick blood smears are most useful for detecting the presence of parasites, because they examine a larger. The clinical pathology laboratory uses the wedge technique for preparation of blood smears. A blood filmor peripheral blood smearis a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. To make a thick blood smear you should take a large drop of blood and spread it to the size of a. This is suitable for the rapid detection of malaria parasites particularly when they are few. These are the most common and accurate malaria tests. A lot of distortion but easier to find parasites list advantages and disadvantages of thin and thick smears for blood parasites cytoplasm is light blue grenn or pink for e. Within a few hours of collecting the blood, the microscopy test can provide valuable information.

Blood films are examined in the investigation of hematological blood disorders and are routinely employed to look for blood. How thick or thin your blood is depends on many factors. The smear is greater than 25 mm long and the feathered edge stops approximately 10 mm from the end of the slide. Apr 20, 2015 blood film for malaria thick and thin blood film hawler medical university college of medicine prepared by. The three main blood cells that the test focuses on are. It is the most commonly used technique for blood examination. Thick blood smear and thin blood smear differs in their definition, uses and sensitivity and these are given below. A field guide for the diagnosis, treatment and prevention.

A blood specimen collected from the patient is spread as a thick or thin blood smear, stained with a romanovsky stain most. Thick and thin blood smears for malaria cs mott childrens. A cls consistantly prepares thin blood smears resulting in minimal counting area. Thick and thin blood smears for malaria cs mott children. These smears had a thickness equivalent to peripheral blood smears, providing an advantage of including the concentration of parasitized rbcs in a thin smear. Thick and thin blood smears for malaria cs mott childrens hospital. Aug 25, 2009 why thick blood protects from a heart attack. Cause of excessively pink or light staininsufficient staining.

Both thick and thin blood smears have their advantages and disadvantages. Thick smears consist of a thick layer of dehemoglobinized lysed red blood cells rbcs. Blood film examination is also important in individuals with thrombocytosis to check for the possibility of a myeloproliferative disorder change such as giant platelets or basophilia. Procedure for thick and thin blood film staining technique. Sep 18, 2017 many dont have any symptoms of thick blood until they experience a blood clot. Blood from a patient with anaemia will have a lower. A blood smear is a diagnostic procedure used in the evaluation and diagnosis of a variety of medical conditions. Thick films contain several layers of red cells, whereas thin. A thick blood smear is said to be a drop of blood on a glass slide. Many dont have any symptoms of thick blood until they experience a blood clot. How to perform a proper thick and thin smear for malaria. Comparative study of peripheral blood smear, quantitative.

Although microscopic examination of thick and thin blood. Blood smears are taken most often from a finger prick. Certain diseases, namely blood cancers, thicken the blood because they lead to abnormally high levels of blood cells. Thick blood smears are most useful for detecting the presence of parasites while thin blood smears. A comparative study on microscopy, rdt and rtpcr for detection of malaria parasites. See dpdx specimen preparation a blood specimen collected from the patient is spread as a thick or thin blood smear, stained with a romanovsky stain most often giemsa, and examined. Common stains used are fields stain, giemsa stain and leishman stain. A thick blood smear is a drop of blood on a glass slide.

Seed haematology the role of the peripheral blood smear in. In an attempt to explain the shortcomings of the routine thickfilm examination in the diagnosis of. More sensitive by 30 times than thin films because. Making and staining a blood smear a wellmade blood smear is a beauty to behold, and likely to yield interesting and significant information for a research project.

Thick blood smear and thin blood smear differs in their definition, uses and sensitivity and these are. Differences between thick blood smear and thin blood smear. Microscopy is an established, relatively simple technique that is familiar to most laboratorians. The team assumes that the results can be transferred to humans and recommends weighing the advantages and disadvantages of.

The well known thin smear methods of blood examination have distinct advantages for purposes of morphologic study. What are the advantages of thin blood smear and thick blood. Thick blood smears are most useful for detecting the presence of parasites. Thick and thin blood smears will let doctors know the percentage of red blood cells that are infected parasite density and what type of parasites are present. But when only a few parasites have been seen in a fresh preparation or a thick film, the thin smear. Thick and thin blood smear for malaria diagnosis learn. A blood filmor peripheral blood smear is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically.

Video tutorial on how to perform a proper thick and thin smear for malaria diagnosis by stephane proux, malaria diagnosis lab supervisor at shoklo malaria research unit smru. Smear sensitivity is further reduced in patients with extrapulmonary tb, those with hivcoinfection, and thosewith disease due to nontuberculous mycobacteria ntm. This method produces a gradual decrease in thickness of the blood from thick to thin ends with the smear terminating in a feathered edge approximately 2 mm long. The team assumes that the results can be transferred to humans and recommends weighing the advantages and disadvantages of anticoagulants. The harvard medical school clearly points out that individuals with thick blood are more prone to being diagnosed with heart disease compared to those with thinner blood. Your hematocrit is a measure of both the number and the size of red blood cells. Thick and thin blood smears for malaria michigan medicine. Prepare a thin and thick smear of the specimen on the same slide which can be done by dividing the slides into two parts and then making the thin smear on one side and thick on the other side. Making and staining a blood smear university of vermont. Advantage, disadvantage and limitations of microscopy to. Thick blood smears are most useful for detecting the presence of parasites while thin blood smears helps doctors discover what species of. In case of a blood check up, i would make a thick smear first to assess the general. Visual criteria are used to detect malaria parasites and to differentiate when possible the various species.

The results are compared with those obtained by pbs thin and thick smears and qbc. Thick blood smear and thin blood smear parasitology online. Nov 08, 2006 twice daily blood samples were taken from day 6 until day 14, then daily until day 21. Without delay, the blood was spread with a glass spreader held at a steep angle to achieve a thick smear. Because the red blood cell is full of endogenous peroxidases and other parasitic enzymes that are. Density is usually assessed by thick films, either by counting parasites per microscope field, or by counting parasites per hundred white blood cells.

What are the advantages of thin blood smear and thick blood smear. The stickiness of platelets the component in blood responsible for clotting problems associated with thick blood. You can prepare thick and thin blood films on separate slides or on the same slide. The heart has more difficulty pumping viscous blood, and thick blood may be more likely to form clots in arteries and veins. Thick blood smear showing trypanosoma species staining the thin and thick blood smear on the same slide with giemsa stain. Is blood like your waistline the thinner, the better. Thick and thin blood smear procedure linkedin slideshare.

Thick blood smear and thin blood smear parasitology. Exposing blood to the foreign object increases the risk of clots that could cause a heart attack, but tissue gradually grows over the stent and lowers that risk. Study 25 terms chapter 10 phlebotomy flashcards quizlet. Normally, peripheral blood is used to prepare smears and depending on the function of the smear, two types of smear can be prepared.

It is a standard procedure that if doctors suspect a disease or infection, a blood smear is typically the first diagnostic test to be performed. A comparative study on microscopy, rdt and rtpcr for. Summary blood film allows for examination of the physical characteristics of the red cells, white cells and platelets under the microscope. The blood clot usually occurs in a persons vein, which can cause pain and affect circulation in and around the. What are the advantages of thin blood smear and thick. Thick films contain 10 to 20 times more blood than thin films, correspondingly providing increased sensitivity for malaria screening. What is difference between thick and thin films of.